Understanding the necessity of regulatory protein machinery in heterologous expression of class-III type of Ocins

Abstract

AbstractTo date, there have been no or just a few reports of successful cloning and expression to create biologically active ocins or bacteriocins. Cloning, expression, and production of class I ocins are problematic because of their structural arrangements, coordinated functions, size, and posttranslational modifications. In the case of class III ocins, there are no reports of obtaining biological active proteins to date. Because of their growing importance, use and rapid functions require understanding mechanistic aspects to obtain biological active protein. As a result, we intend to clone and express the class III type. Also, by fusion or chimaera, they reshape class I types that lack posttranslational modifications into class III. Therefore, this construct resembles a class III type ocin. With the exception of Zoocin, expression of the proteins was found to be physiologically ineffective after cloning. But, few cell morphological changes such as elongation, aggregation, and the formation of terminal hyphae were observed. However, it was discovered that the target indicator had been altered toVibrio spp. in a few. Finally, we confirm the existence of unidentified additional intrinsic factors for succesful expression to obtain biologically active protein.