Optimization of an antiStaphylococcusantibiotic produced by tropical soil dwellingStreptomyces parvulus

Abstract

AbstractAn antibiotic produced by strainStreptomyces parvulusshowing activity againstStaphylococcus citreuswas subjected to various optimization parameters for enhancing its production. Nutritional and physiological parameters produced byS. parvulusunder shaken flask conditions were determined. Optimization of these parameters led to 11% increase in antibiotic activity with a mean zone of inhibition of 42 mm.Highest antibiotic production was obtained at 250 rpm for 14 days with optimum temperature of 28°C and pH 7. Kuster#x2019;s modified medium containing glycerol 0.7% (v/v), casein 0.03% (w/v), NaCl 0% (w/v), phosphate 0.25% (w/v), KNO3 0.1% (w/v) and CaCO3 0.0015% (w/v) concentration was found ideal.