Sequence Analysis and Homology Modelling of SmHQT Protein, a Key Player in Chlorogenic Acid Pathway of Eggplant-Reference-Cited by-同舟云学术

Sequence Analysis and Homology Modelling of SmHQT Protein, a Key Player in Chlorogenic Acid Pathway of Eggplant

Published:2019-04-04 Issue: Volume: Page:
ISSN:
Container-title:
language:
Short-container-title:

Author:

Kaushik Prashant,Saini Dinesh Kumar

Abstract

AbstractEggplant is an important vegetable that belongs to family Solanaceae. Fruits of eggplant are rich in phenolic acids. Chlorogenic acid makes up to 90 per cent of total phenolic acids present in the eggplants fruit flesh. Eggplant hydroxycinnamoyl CoA-quinate (SmHQT) is the central enzyme that modulates the last step of the chlorogenic acid pathway of eggplant. Here, we have analysed the sequence of eggplant SmHQT protein in eggplant. The sequence obtained from the NCBI was aligned using MUSCLE. After that, homology modelling was performed using MODELLER 9v15. Model with Dope Z-Score of −1.596 was selected and verified for viability under real conditions using several online tools. Also, the docking was performed with this model. Overall, this could be useful in developing eggplant varieties rich in phenolic acids especially chlorogenic acid.

Publisher

Cold Spring Harbor Laboratory

Reference41 articles.

1. catRAPID omics: a web server for large-scale prediction of protein-RNA interactions

2. Extensive feature detection of N-terminal protein sorting signals

3. Toward the estimation of the absolute quality of individual protein structure models;Bioinformatics,2010

4. HIV-1 Vif binds to APOBEC3G mRNA and inhibits its translation

5. Bittrich, S. , Heinke, F. and D. Labudde , 2015. eQuant-A Server for Fast Protein Model Quality Assessment by Integrating High-Dimensional Data and Machine Learning. pp. 419–433. Beyond Databases, Architectures and Structures. Advanced Technologies for Data Mining and Knowledge Discovery, Springer, Cham

Cited by 4 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Biochemical Composition of Eggplant Fruits: A Review;Applied Sciences;2021-07-30

2. Computational insights into the identification of a potent matrix metalloproteinase inhibitor from Indigofera aspalathoides to control cancer metastasis;3 Biotech;2021-04-07

3. Computational exploration of vicine – an alkaloid glycoside mediated pathological hallmark of adenosine kinase to promote neurological disorder;Metabolic Brain Disease;2021-01-26

4. A system-level approach to investigate alloxan-induced toxicity in microtubule-binding protein to lead type 2 diabetes mellitus;Molecular Diversity;2020-03-26