Engineered acetoacetate-inducible whole-cell biosensors based on the AtoSC two-component system

Author:

Rutter Jack W.,Dekker Linda,Fedorec Alex J. H.ORCID,Gonzales David T.,Wen Ke Yan,Tanner Lewis E. S.,Donovan Emma,Ozdemir Tanel,Thomas Geraint,Barnes Chris P.ORCID

Abstract

AbstractWhole-cell biosensors hold potential in a variety of industrial, medical and environmental applications. These biosensors can be constructed through the repurposing of bacterial sensing mechanisms, including the common two-component system. Here we report on the construction of a range of novel biosensors that are sensitive to acetoacetate, a molecule that plays a number of roles in human health and biology. These biosensors are based on the AtoSC two-component system. An ODE model to describe the action of the AtoSC two-component system was developed and sensitivity analysis of this model used to help inform biosensor design. The final collection of biosensors constructed displayed a range of switching behaviours, at physiologically relevant acetoacetate concentrations and can operate in several Escherichia coli host strains. It is envisaged that these biosensor strains will offer an alternative to currently available commercial strip tests and, in future, may be adopted for more complex in vivo or industrial monitoring applications.

Publisher

Cold Spring Harbor Laboratory

Reference50 articles.

1. Synthetic Biology Enables Programmable Cell-Based Biosensors;ChemPhysChem,2020

2. Bacterial transmembrane signalling systems and their engineering for biosensing

3. Baojun Wang , Maggie Hicks , and Till T. Bachmann . Synthetic Biology Enables Programmable Cell-Based Biosensors. ChemPhysChem, page cphc.201900739, oct 2019.

4. Detection of pathological biomarkers in human clinical samples via amplifying genetic switches and logic gates

5. Development of a whole cell biosensor for the detection of inorganic mercury;Environmental Technology & Innovation,2017

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3