Proteomic Analysis ofPlasmodiumMerosomes: The Link Between Liver and Blood Stages in Malaria

Abstract

SummaryThe pre-erythrocytic liver stage of the malaria parasite, comprising sporozoites and the liver stages into which they develop, remains one of the least understood parts of the lifecycle, in part owing to the low numbers of parasites. Nonetheless, it is recognized as an important target for anti-malarial drugs and vaccines. Here we provide the first proteomic analysis of merosomes, which define the final phase of the liver stage and are responsible for initiating the blood stage of infection. We identify a total of 1879 parasite proteins, and a core set of 1188 proteins quantitatively detected in every biological replicate, providing an extensive picture of the protein repertoire of this stage. This unique dataset will allow us to explore key questions about the biology of merosomes and hepatic merozoites.HighlightsFirst proteome of the merosome stage of malaria parasitesQuantitative detection of 1188 parasite proteins across 3 biological replicatesComparison to blood stage proteomes identifies shared and unique proteinsDiscovery of cleaved PEXEL motifs highlights liver stage protein exportIn BriefThe merosome stage that links malaria liver and blood stage infection is poorly understood. Here we provide the first proteome of this life cycle stage using thePlasmodium bergheirodent malaria model.Graphical Abstract