Characterization of the novelSmsHSP24.1Promoter: Unveiling its abiotic stress-inducible expression in transgenic Eggplant (Solanum melongenaL.)

Abstract

AbstractPromoters play a pivotal role in regulating gene expression, orchestrating vital processes in plants, including their responses to various environmental stresses. In this study, we focus on the comprehensive characterization of theSmsHSP24.1promoter, a novelcis-acting element, within the context of transgenic Eggplant (Solanum melongenaL.). This detailed analysis shed light on the intricate mechanisms governingSmsHSP24.1promoter-driven gene regulation, particularly in response to adverse environmental challenges such as heat, salt and drought stressors offering valuable insights into its role in plant stress adaptation. The advances in our understanding of promoter-driven gene regulation also contribute to the broader goal of enhancing crop resilience to abiotic stresses, positioning theSmsHSP24.1promoter as a promising tool in agricultural biotechnology applications.Highlights:Demonstrated that the full-length 2.0 kb SmsHSP24.1 promoter significantly enhances gene expression under heat stress, with an observable decline in expression with promoter truncation.Identified specific regulatory elements within the SmsHSP24.1 promoter that are decisive for inducible expression in response to abiotic stresses such as heat, salt, and drought.Highlighted the utility of the SmsHSP24.1 promoter in crop improvement programs, offering a tool for developing transgenic plant tolerance to combined stresses.