Gastric digestion and changes in serum amino acid concentrations after consumption of casein from cow and goat milk: a randomized crossover trial in healthy men

Abstract

ABSTRACTBackgroundIn vitrostudies show that goat milk proteins form less compact coagulates in the stomach compared to cow milk proteins, which may facilitate gastric digestion and amino acid (AA) absorption. However, this has not been confirmedin vivoin humans.ObjectiveTo examine gastric digestion and changes in AA concentrations after cow milk-derived (cow MC) and goat milk-derived casein (goat MC) ingestion.MethodsIn this single-blind randomized cross-over study 18 men (age 23 ± 1.6 years, BMI 23 ± 1.6 kg/m2) consumed 300 ml of a drink containing 30 g of cow MC or goat MC. Participants underwent gastric MRI scans at baseline and every 10 minutes up to 60 minutes postprandially. Blood was drawn at baseline and up to 4 hours postprandially. In addition, participants verbally rated their appetite after each MRI measurement. Primary outcomes were gastric emptying and AA concentrations. Secondary outcome was gastric coagulation as inferred by image texture metrics.ResultsGastric emptying half-time was 80 ± 25 minutes for goat and 85 ± 24 minutes for cow MC (p = 0.395). In line with this, gastric emptying of the drinks over time was similar (MD 0.77 ml, 95% CI [-6.9, 8.5], p=0.845). Serum essential AA (MD -110 µmol/L, 95% CI [-162, -58]) was higher over time for cow MC (p<0.001). The image texture metric contrast was lower for cow MC (MD 0.010, 95% CI [0.001, 0.020], p=0.036).ConclusionCow MC and goat MC have different coagulating properties, as inferred by AA concentrations and supported by image texture analysis. This did not influence overall gastric emptying or the emptying of the liquid and coagulated fractions, which were similar. This warrants furtherin vivoresearch on casein coagulation in the food matrix to help determine the optimal use for cow and goat milk and their protein fractions.Financial support: the study was funded by Ausnutria Dairy Corporation Ltd.Clinical trial registry number: NL8137 (Netherlands Trial Registry), accessible throughhttps://trialsearch.who.int/Trial2.aspx?TrialID=NL-OMON28580