Flynotyper 2.0: An updated tool for rapid quantitative assessment ofDrosophilaeye phenotypes

Abstract

AbstractAbout two-thirds of the genes in theDrosophila melanogastergenome are also involved in its eye development, making theDrosophilaeye an ideal system for genetic studies. We previously developed Flynotyper, a software that uses image processing operations to identify and quantify the degree of roughness by measuring disorderliness of ommatidial arrangement in the fly eye. This software has enabled researchers to quantify morphological defects of thousands of eye images caused by genetic perturbations. Here, we present Flynotyper 2.0, a software that has an updated computer vision library, improved performance, and a streamlined pipeline for high-throughput analysis of multiple eye images. We also tested several batches ofDrosophilaeye images to ensure robustness and reproducibility of the updated Flynotyper 2.0 software.Availability and implementationThe source code for Flynotyper 2.0 can be downloaded and installed fromhttps://github.com/girirajanlab/flynotyper-desktop-application.