Tissue and cellular spatiotemporal dynamics in colon aging

Author:

Daly Aidan C.ORCID,Cambuli Francesco,Äijö Tarmo,Lötstedt Britta,Marjanovic Nemanja,Kuksenko Olena,Smith-Erb Matthew,Fernandez Sara,Domovic Daniel,Van Wittenberghe Nicholas,Drokhlyansky Eugene,Griffin Gabriel K,Phatnani Hemali,Bonneau Richard,Regev Aviv,Vickovic Sanja

Abstract

ABSTRACTTissue structure and molecular circuitry in the colon can be profoundly impacted by systemic age-related effects, but many of the underlying molecular cues remain unclear. Here, we built a cellular and spatial atlas of the colon across three anatomical regions and 11 age groups, encompassing ∼1,500 mouse gut tissues profiled by spatial transcriptomics and ∼400,000 single nucleus RNA-seq profiles. We developed a new computational framework, cSplotch, which learns a hierarchical Bayesian model of spatially resolved cellular expression associated with age, tissue region, and sex, by leveraging histological features to share information across tissue samples and data modalities. Using this model, we identified cellular and molecular gradients along the adult colonic tract and across the main crypt axis, and multicellular programs associated with aging in the large intestine. Our multi-modal framework for the investigation of cell and tissue organization can aid in the understanding of cellular roles in tissue-level pathology.

Publisher

Cold Spring Harbor Laboratory

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