Tiny amphibious insects use tripod gait for seamless transition across land, water, and duckweed

Abstract

AbstractInsects exhibit remarkable adaptability in their locomotive strategies across diverse environments, a crucial trait for foraging, survival, and predator avoidance.Microvelia, tiny 2-3 mm insects that adeptly walk on water surfaces, exemplify this adaptability by using the alternating tripod gait in both aquatic and terrestrial terrains. These insects commonly inhabit low-flow ponds and streams cluttered with natural debris like leaves, twigs, and duckweed. Using high-speed imaging and pose-estimation software, we analyzeMicrovelia spp.’smovement across water, sandpaper (simulating land), and varying duckweed densities (10%, 25%, and 50% coverage). Our results revealMicroveliamaintain consistent joint angles and strides of their upper and hind legs across all duckweed coverages, mirroring those seen on sandpaper.Microveliaadjust the stride length of their middle legs based on the amount of duckweed present, decreasing with increased duckweed coverage and at 50% duckweed coverage, their middle legs’ strides closely mimic their strides on sandpaper. Notably,Microveliaachieve speeds up to 56 body lengths per second on water, nearly double those observed on sandpaper and duckweed (both rough, frictional surfaces), highlighting their higher speeds on low friction surfaces such as the water’s surface. This study highlightsMicrovelia’s ecological adaptability, setting the stage for advancements in amphibious robotics that emulate their unique tripod gait for navigating complex terrains.