First endangered black-footed ferrets,Mustela nigripes, cloned for genetic rescue

Author:

Novak Ben J.,Gober Pete,Bortner Robyn,Garelle Della,Wright Mary,Chuven Justin,Houck Marlys L.,Ryder Oliver A.,Milutinovich Dennis,Benavidez Jill,Ryan Kerry,Walker Shawn,Arenivas Sanaz Sadeghieh,Aston Lauren,Russell Blake,Marinari Paul,Crosier Adrienne,Helmick Kelly,Gibson Mary R.,Carlsen Daniel P.,Swanson Bradley J.,Wisely Samantha M.,White Zoe S.,Lynch Colleen,Phelan Ryan

Abstract

AbstractAn endangered black-footed ferret female that died in 1988 with no living descendants in the current population was successfully cloned from cryopreserved cells using cross-species somatic cell nuclear transfer, producing three healthy kits. Incorporating progeny from these clones would provide an 8th founder to the breeding program and increase genetic variation to the species’ limited gene-pool. This marks the first time a native U.S. endangered species has been cloned.

Publisher

Cold Spring Harbor Laboratory

Reference25 articles.

1. A Road Map for 21st Century Genetic Restoration: Gene Pool Enrichment of the Black-Footed Ferret

2. Marinari, P. and Lynch, C. AZA SAFE Program Population Analysis and Breeding and Transfer Plan for Black-footed Ferret (Mustela nigripes). AZA Population Management Center: Chicago, IL, USA. (2023).

3. Santymire, R. & Graves, G. Black-footed Ferret SAFE Program Action Plan 2019-2021. 1–26 (2019).

4. Inbreeding causes decreased seminal quality affecting pregnancy and litter size in the endangered black-footed ferret;Anim. Conserv,2019

5. The alluring simplicity and complex reality of genetic rescue

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