Making Plants Smell Like Moths: Nicotiana benthamiana release moth pheromone alcohol, aldehyde and acetate upon transient expression of biosynthetic genes of different origin

Author:

Xia Yi-HanORCID,Ding Bao-Jian,Dong Shuang-Lin,Wang Hong-Lei,Hofvander Per,Löfstedt ChristerORCID

Abstract

AbstractUsing genetically modified plants as natural dispensers of insect pheromones may eventually become part of a novel strategy for integrated pest management. In the present study, we first characterized essential functional genes for sex pheromone biosynthesis in the rice stem borer Chilo suppressalis (Walker) by heterologous expression in Saccharomyces cerevisiae and Nicotiana benthamiana, including two desaturase genes CsupYPAQ and CsupKPSE, and a reductase gene CsupFAR2. Subsequently, we co-expressed CsupYPAQ and CsupFAR2 together with the previously characterized moth desaturase AtrΔ11 in N. benthamiana. This resulted in the production of (Z)-11-hexadecenol together with (Z)-11-hexadecenal, the major pheromone component of C. suppressalis. Both compounds were collected from the transformed N. benthamiana headspace volatiles using solid phase microextraction. We finally added the expression of a yeast acetyltransferase gene ATF1 and could then confirm also (Z)-11-hexadecenyl acetate release from the plant. Our results pave the way for stable transformation of plants to be used as biological pheromone sources in different pest control strategies.

Publisher

Cold Spring Harbor Laboratory

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Trends in the study of insect sex pheromones and their use in pest management;Journal of Japan Association on Odor Environment;2022-01-25

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