Author:
Koldobskiy Michael A.,Camacho Oscar,Reddy Pradeep,Izpisua Belmonte Juan Carlos,Feinberg Andrew P.
Abstract
ABSTRACTRejuvenation of tissues in physiologically aging mice can be accomplished by long-term partial reprogramming via expression of reprogramming factors (Oct4, Sox2, Klf4 and c-Myc). To investigate the epigenetic determinants of partial reprogramming-mediated rejuvenation, we used whole genome bisulfite sequencing to carry out unbiased comprehensive profiling of DNA methylation changes in skin from mice subjected to partial reprogramming, as well as young and untreated old controls. We found a striking convergence of age- and rejuvenation-related epigenetic alterations on targets of the Polycomb repressive complex 2 (PRC2). These results are also supported by a likewise prominent enrichment of PRC2 targets in gene expression data, suggesting that PRC2 activity can modulate aging and mediate tissue rejuvenation.
Publisher
Cold Spring Harbor Laboratory
Reference14 articles.
1. In Vivo Amelioration of Age-Associated Hallmarks by Partial Reprogramming
2. Reprogramming to recover youthful epigenetic information and restore vision
3. In Vivo Reprogramming Ameliorates Aging Features in Dentate Gyrus Cells and Improves Memory in Mice;Stem Cell Reports,2020
4. In vivo partial reprogramming of myofibers promotes muscle regeneration by remodeling the stem cell niche;Nature Communications 2021 12:1,2021
5. In vivo partial reprogramming alters age-associated molecular changes during physiological aging in mice;Nature Aging 2022 2:3,2022
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