High genomic plasticity and unique features ofXanthomonas translucenspv.graminisrevealed through comparative analysis of complete genome sequences

Abstract

AbstractBackgroundXanthomonas translucenspv.graminis(Xtg) is a major bacterial pathogen of economically important forage grasses, causing severe yield losses. So far, genomic resources for this pathovar consisted mostly of draft genome sequences, and only one complete genome sequence was available, preventing comprehensive comparative genomic analyses. Such comparative analyses are essential in understanding the mechanisms involved in the virulence of pathogens and to identify virulence factors involved in pathogenicity.ResultsIn this study, we produced high-quality, complete genome sequences of four strains ofXtg, complementing the recently obtained complete genome sequence of theXtgpathotype strain. These genomic resources allowed for a comprehensive comparative analysis, which revealed a high genomic plasticity with many chromosomal rearrangements, although the strains were highly related, with 99.9 to 100% average nucleotide identity. A high number of transposases were exclusively found inXtgand corresponded to 413 to 457 insertion/excision transposable elements per strain. These mobile genetic elements are likely to be involved in the observed genomic plasticity and may play an important role in the adaptation ofXtg. The pathovar was found to lack a type IV secretion system, and it possessed the smallest set of type III effectors in the species. However, three XopE and XopX family effectors were found, while in the other pathovars of the species two or less were present. Additional genes that were specific to the pathovar were identified, including a unique set of minor pilins of the type IV pilus, 17 TonB-dependent receptors (TBDRs), and 11 degradative enzymes.ConclusionThese results suggest a high adaptability ofXtg, conferred by the abundance of mobile genetic elements, which may have led to the loss of many features. Conserved features that were specific toXtgwere identified, and further investigation will help to determine genes that are essential to pathogenicity and host adaptation ofXtg.