Mechanisms of cilia regeneration inXenopusmulticiliated epitheliumin vivo

Abstract

SUMMARYCilia regeneration is a physiological event, and while studied extensively in unicellular organisms, it remains poorly understood in vertebrates. In this study, usingXenopusmulticiliated cells (MCCs) as a model, we demonstrate that, unlike unicellular organisms, deciliation removes the transition zone (TZ) along with the ciliary axoneme. While MCCs immediately begin the regeneration of the ciliary axoneme, surprisingly, the assembly of TZ was delayed. Instead, ciliary tip proteins, Sentan and Clamp, were the first to localize to regenerating cilia. Using cycloheximide (CHX) to block new protein synthesis, we show that the TZ protein B9d1 is not a component of the cilia precursor pool and requires new transcription/translation providing insights into the delayed repair of TZ. Moreover, CHX treatment led MCCs to assemble fewer (∼ ten compared to ∼150 in controls) but about wild-type length (78% of WT) cilia by gradually concentrating ciliogenesis proteins like IFT43 at a select few basal bodies, highlighting the exciting possibility of protein transport between basal bodies to facilitate faster regeneration in cells with multiple cilia. In summary, we demonstrate that MCCs begin regeneration with the assembly of ciliary tip and axoneme followed by TZ, questioning the importance of TZ in motile ciliogenesis.