Author:
Liu Ge,Program NISC Comparative Sequencing,Zhao Shaying,Bailey Jeffrey A.,Sahinalp S. Cenk,Alkan Can,Tuzun Eray,Green Eric D.,Eichler Evan E.
Abstract
We performed a detailed analysis of both single-nucleotide and large insertion/deletion events based on large-scale comparison of 10.6 Mb of genomic sequence from lemur, baboon, and chimpanzee to human. Using a human genomic reference, optimal global alignments were constructed from large (>50-kb) genomic sequence clones. These alignments were examined for the pattern, frequency, and nature of mutational events. Whereas rates of single-nucleotide substitution remain relatively constant (1–2 × 10−9 substitutions/site/year), rates of retrotransposition vary radically among different primate lineages. These differences have lead to a 15%–20% expansion of human genome size over the last 50 million years of primate evolution, 90% of it due to new retroposon insertions. Orthologous comparisons with the chimpanzee suggest that the human genome continues to significantly expand due to shifts in retrotransposition activity. Assuming that the primate genome sequence we have sampled is representative, we estimate that human euchromatin has expanded 30 Mb and 550 Mb compared to the primate genomes of chimpanzee and lemur, respectively.[Supplemental material is available online at www.genome.org.]
Publisher
Cold Spring Harbor Laboratory
Subject
Genetics(clinical),Genetics
Reference51 articles.
1. Alkan C. Tuzun E. Eicher E.E. Bailey J.A. Sahinalp S.C. (2002) MaM: Multiple alignment manipulator. Currents in computational molecular biology 2002 . (Celera Genomics, Rockville, MD), pp 3–4.
2. Whole-Genome Shotgun Assembly and Analysis of the Genome of
Fugu rubripes
3. Recent Segmental Duplications in the Human Genome
4. Molecular evolution of the ψ η-globin gene locus: Gibbon phylogeny and the hominoid slowdown.;Bailey;Mol. Biol. Evol.,1991
5. Alu repeats and human genomic diversity
Cited by
116 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献