Abstract
AbstractRodents are the main animal model to study sleep. Yet, in spite of a large consensus on the distinction between rapid-eye-movements sleep (REM) and non-REM sleep (NREM) in both humans and rodent, there is still no equivalent in mice of the NREM subdivision classically described in humans.Here we propose a classification of sleep stages in mice, inspired by human sleep scoring. By using chronic recordings in medial prefrontal cortex (mPFC) and hippocampus we can classify three NREM stages with a stage N1 devoid of any low oscillatory activity and N3 with a high density of delta waves. These stages displayed the same evolution observed in human during the whole sleep or within sleep cycles. Importantly, as in human, N1 in mice is the first stage observed at sleep onset and is increased after sleep fragmentation in Orexin-/- mice, a mouse model of narcolepsy.We also show that these substages are associated to massive modification of neuronal activity. Moreover, considering these stages allows to predict mPFC neurons evolution of firing rates across sleep period. Notably, neurons preferentially active within N3 decreased their activity over sleep while the opposite is seen for those preferentially active in N1 and N2.Overall this new approach shows the feasibility of NREM sleep sub-classification in rodents, and, in regard to the similarity between sleep in both species, will pave the way for further studies in sleep pathologies given the perturbation of specific sleep substages in human pathologies such as insomnia, somnambulism, night terrors, or fibromyalgia.
Publisher
Cold Spring Harbor Laboratory
Reference70 articles.
1. A model of human sleep homeostasis based on EEG slow-wave activity: Quantitative comparison of data and simulations
2. American Psychiatric Association. Diagnostic and Statistical Manual of Mental Disorders (DSM-5®) [Internet]. American Psychiatric Publishing; 2013. Available from: https://books.google.fr/books?id-JivBAAAQBAJ
3. Bagur S , Lacroix MM , De Lavilléon G , Lefort J , Geoffroy H , Benchenane K . Harnessing olfactory bulb oscillations to perform fully brain-based sleep scoring and track sleep/wake transitions across multiple time scales in mice. PLoS Biol.
4. Über den zeitlichen verlauf der negativen schwankung des nervenstroms;Arch Ges Physiol,1929
5. NREM sleep with low-voltage EEG in the rat;Sleep,1987
Cited by
11 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献