Author:
Kowarsky Mark,Anselmi Chiara,Hotta Kohji,Burighel Paolo,Zaniolo Giovanna,Caicci Federico,Rosental Benyamin,Neff Norma F,Ishizuka Katherine J,Palmeri Karla J,Okamoto Jennifer,Gordon Tal,Weissman Irving L,Quake Stephen R,Manni Lucia,Voskoboynik Ayelet
Abstract
SummaryAll chordates, including urochordates such as tunicates, develop through embryogenesis. The chordate larvae of colonial tunicates metamorphose to lose all chordate structures such as notochord, neural tube, segmented musculature, and then develop by asexual reproduction [blastogenesis], whereby stem cells form tissues and organs. These two developmental pathways establish the same body axis, morphogenetic patterning and organ formation. It is unknown if this convergent morphology implies convergent cellular and molecular mechanisms, and whether the stem cells that mediate these processes differ. Using the colonial tunicate Botryllus schlosseri, we combined transcriptome sequencing and multiple microscopy techniques to study the molecular and morphological signatures of cells at each developmental stage of embryogenesis and blastogenesis. This revealed that the molecular programs are distinct, but the blastogenic tissue-specific stem cells and embryonic precursor populations share similar molecular profiles. By comparing embryogenesis in other chordates we found shared developmental principles, highlighting transcription factors as key evolutionary conserved elements. This study establishes a platform for advancing the science of stem cell biology and regulation of development and regeneration.
Publisher
Cold Spring Harbor Laboratory
Reference93 articles.
1. HTSeq--a Python framework to work with high-throughput sequencing data
2. Expression of the homophilic adhesion molecule, Ep-CAM, in the mammalian germ line
3. Ballarin, L. , and Cima, F . (2005). Cytochemical properties of Botryllus schlosseri haemocytes: indications for morpho-functional characterisation. European Journal of Histochemistry 255–264.
4. A tale of death and life: natural apoptosis in the colonial ascidian Botryllus schlosseri (Urochordata;Ascidiacea). Current pharmaceutical design,2008
5. GeneAnalytics: An Integrative Gene Set Analysis Tool for Next Generation Sequencing;RNAseq and Microarray Data. OMICS: A Journal of Integrative Biology,2016
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献