Author:
Hopp Christine S.,Kanatani Sachie,Archer Nathan K.,Miller Robert J.,Liu Haiyun,Chiou Kevin,Miller Lloyd S.,Sinnis Photini
Abstract
AbstractMalaria infection starts with the injection of motilePlasmodiumsporozoites into the host’s skin during a mosquito bite. Previous studies using the rodent malaria model indicate that the dermal inoculation site may be where sporozoites are most vulnerable to antibodies, yet, functionalin vivoassays with human malaria parasites are lacking. Here, we present the first characterization ofP. falciparumsporozoites in the skin, comparing their motility to two rodent malaria species and investigating whether the environment of its natural host influencesP. falciparumsporozoite motility using a human skin xenograft model. The combined data suggest that in contrast to the liver and blood stages, the skin is not a species-specific barrier forPlasmodium. We observe thatP. falciparumsporozoites inoculated into mouse skin move with similar speed, displacement and duration, and enter blood vessels in similar numbers as the rodent parasites. Thus, interventions targetingP. falciparumsporozoite migration can be tested in the murine dermis. Importantly, to streamline quantification of sporozoite motility, we developed a toolbox allowing for automated detection and tracking of sporozoites in intravital microscopy videos. This establishes a platform to test vaccine candidates, immunization protocols, monoclonal antibodies and drug candidates for their impact on human malaria sporozoitesin vivo. Screening of intervention strategies forin vivoefficacy againstPfsporozoites using this new platform will have the potential to validate targets prior to expensive clinical trials.
Publisher
Cold Spring Harbor Laboratory
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献