Abstract
AbstractMicroRNAs (miRNAs) are a class of small non-coding RNAs involved in many biological processes, including the immune pathways that control bacterial, parasitic, and viral infections. Pathogens probably modify host miRNAs to facilitate successful infection, so they might be useful targets for vaccination strategies. There are little data on differentially expressed miRNAs in the black- legged tick Ixodes scapularis after infection with Borrelia burgdorferi, the causative agent of Lyme disease in the United States. Small RNA sequencing and qRT-PCR analysis were used to identify and validate differentially-expressed I. scapularis salivary miRNAs. Small RNA-seq yielded 133,465,828 (≥18 nucleotides) and 163,852,135 (≥18 nucleotides) small RNA reads from Borrelia- infected and uninfected salivary glands for downstream analysis using the miRDeep2 algorithm. Two hundred and fifty-four miRNAs were identified across all datasets, 25 of which were high confidence and 51 low confidence known miRNAs. Twenty-three miRNAs were differentially expressed in uninfected and infected salivary glands: 11 were upregulated and 12 were downregulated upon pathogen infection. This study provides new insights into the miRNAs expressed in I. scapularis salivary glands and pave the way for their functional manipulation to prevent or treat B. burgdorferi infection.
Publisher
Cold Spring Harbor Laboratory
Cited by
1 articles.
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