Venezuelan equine encephalitis complex, Madariaga and Eastern equine encephalitis viruses genome detection in human and mosquito populations
Author:
Carrera Jean-PaulORCID, Araúz Dimelza, Rojas Alejandra, Cardozo Fátima, Stittleburg Victoria, Claro Ingra Morales, Galue Josefrancisco, Lezcano-Coba Carlos, Rebello Moreira Filipe Romero, Felipe-Rivera Luis, Chen-Germán Maria, Moreno Brechla, Capitan-Barrios Zeuz, López-Vérges Sandra, Pascale Juan Miguel, Sabino Ester C.ORCID, Valderrama Anayansi, Hanley Kathryn A., Donnelly Christl A.ORCID, Vasilakis Nikos, Faria Nuno R., Waggoner Jesse J.
Abstract
AbstractEastern equine encephalitis virus (EEEV), Madariaga virus (MADV) and Venezuelan equine encephalitis virus complex (VEEV) are New World mosquito-borne alphaviruses and cause severe neurological disease in human and equine hosts. However, their detection during the acute phase is complicated by non-specific clinical manifestations and lack of available diagnostic tools. To develop and clinically evaluate rRT-PCRs for VEEV complex, MADV and EEEV, primers and probes were designed from publicly available whole-genome sequences. The rRT-PCRs were validated using 15 retrospective serum samples from febrile patients collected during the 2015 and 2017 alphavirus outbreaks in Panama. In addition, the protocol was validated with 150 mosquito pools from 2015, and with 118 samples from prospective disease surveillance from 2021 and 2022. The rRT-PCRs detected VEEV complex RNA in 10 samples (66.7%) from the 2015 and 2017 outbreaks, and in one of these ten samples, both VEEV complex and MADV RNAs were detected. Additionally, VEEV complex RNA was detected in 5 suspected dengue from prospective disease surveillance. The rRT-PCR assays detected VEEV complex RNA in 3 fromCulex(Melanoconion)vomeriferpools, 2 of which yielded VEEV isolates. Untargeted sequencing and phylogenetic analysis identified VEEV ID subtype in seven VEEV complex RNA positive sample. The VEEV complex, MADV and EEEV rRT-PCRs provide accurate detection while yielding significant benefits over currently available molecular methods. Our results suggest that 11.9% of suspected dengue cases in Panama are VEEV infections.
Publisher
Cold Spring Harbor Laboratory
Reference43 articles.
1. Navarro JC , Carrera JP , Liria J , Auguste AJ , Weaver SC . 2017. Alphaviruses in Latin America and the introduction of chikungunya virusHuman Virology in Latin America: From Biology to Control. 2. Aguilar P v. , Estrada-Franco JG , Navarro-Lopez R , Ferro C , Haddow AD , Weaver SC . 2011. Endemic Venezuelan equine encephalitis in the Americas: Hidden under the dengue umbrella. Future Virol https://doi.org/10.2217/fvl.11.50. 3. Arrigo NC , Adams AP , Weaver SC . 2010. Evolutionary Patterns of Eastern Equine Encephalitis Virus in North versus South America Suggest Ecological Differences and Taxonomic Revision. J Virol https://doi.org/10.1128/jvi.01586-09. 4. Forshey BM , Guevara C , Laguna-Torres VA , Cespedes M , Vargas J , Gianella A , Vallejo E , Madrid C , Aguayo N , Gotuzzo E , Suarez V , Morales AM , Beingolea L , Reyes N , Perez J , Negrete M , Rocha C , Morrison AC , Russell KL , Blair PJ , Olson JG , Kochel TJ . 2010. Arboviral etiologies of acute febrile illnesses in western south America, 2000-2007. PLoS Negl Trop Dis 4. 5. Carrera J-P , Forrester N , Wang E , Vittor AY , Haddow AD , López-Vergès S , Abadía I , Castaño E , Sosa N , Báez C , Estripeaut D , Díaz Y , Beltrán D , Cisneros J , Cedeño HG , Travassos da Rosa AP , Hernandez H , Martínez-Torres AO , Tesh RB , Weaver SC . 2013. Eastern Equine Encephalitis in Latin America. New England Journal of Medicine https://doi.org/10.1056/nejmoa1212628.
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|