Abstract
ABSTRACTBrain organoids are important models for mimicking some three-dimensional (3D) cytoarchitectural and functional aspects of the brain. Multielectrode arrays (MEAs) that enable recording and stimulation of activity from electrogenic cells are widely utilized in biomedical engineering. However, conventional MEAs, initially designed for monolayer cultures, offer limited recording contact area restricted to the bottom of the 3D organoids. Inspired by the shape of electroencephalography (EEG) caps, we developed miniaturized chip-integrated MEA caps for organoids. The optically transparent shells are composed of self-folding polymer leaflets with conductive polymer-coated metal electrodes. Tunable folding of the minicaps’ polymer leaflets guided by mechanics simulations enables versatile recording from organoids of different sizes and we validate the feasibility of electrophysiology recording from 400-600 µm sized organoids for up to four weeks and in response to glutamate stimulation. Our studies suggest that 3D shell MEAs offer significant potential for high signal-to-noise and 3D spatiotemporal brain organoid recording.
Publisher
Cold Spring Harbor Laboratory
Reference46 articles.
1. Thoughts on limitations of animal models
2. The use of brain organoids to investigate neural development and disease
3. Complex Oscillatory Waves Emerging from Cortical Organoids Model Early Human Brain Network Development;Cell Stem Cell,2019
4. Biology-inspired microphysiological systems to advance patient benefit and animal welfare in drug development;ALTEX,2020
5. MPS-WS Berlin 2019, Human microphysiological systems for drug development;Science,2021