Author:
Vostrosablin Nikita,Lim Shuhui,Gopal Pooja,Brazdilova Kveta,Parajuli Sushmita,Wei Xiaona,Gromek Anna,Spale Martin,Muzdalo Anja,Yeo Constance,Wardyn Joanna,Mejzlik Petr,Henry Brian,Partridge Anthony W.,Bitton Danny A.
Abstract
AbstractRecent COVID-19 vaccines unleashed the potential of mRNA-based therapeutics. mRNA optimization is indispensable for reducing immunogenicity, ensuring stability, and maximizing protein output. We present mRNAid, an experimentally validated software for mRNA optimization and visualization that generates mRNA sequences with comparable if not superior characteristics to commercially optimized sequences. To encompass all aspects of mRNA design, we also interrogated the impact of uridine content, nucleoside analogs and UTRs on expression and immunogenicity.
Publisher
Cold Spring Harbor Laboratory
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