Computational modeling of molecular structure of microRNA inhibitors selected against microRNA over-expressed in thyroid cancer

Abstract

ABSTRACTIntroductionThyroid cancer is the most prevalent malignant neoplasm of endocrine system and advances in thyroid molecular biology studies demonstrate that microRNAs (miRNAs) seem to play a fundamental role in tumor triggering and progression. The miRNAs inhibitors are nucleic acid-based molecules that blockade miRNAs function, making unavailable for develop their usual function, also acting as gene expression controlling molecules.ObjectiveTo develop in silico projection of molecular structure of miRNA inhibitors against miRNA over-expressed in thyroid cancer.MethodsWe conducted a search of the nucleotide sequence of 12 miRNAs already defined as inhibitors against miRNA over-expressed in thyroid cancer, realizing in silico projection of the molecular structure of following miRNAs: miRNA-101, miRNA-126, miRNA-126-3p, miRNA-141, miRNA-145, miRNA-146b, miRNA-206, miRNA-3666, miRNA-497, miRNA-539, miRNA-613, and miRNA-618. The nucleotides were selected using GenBank that is the NIH genetic sequence database. The sequences obtained were aligned with the Clustal W multiple alignment algorithms. For the molecular modeling, the structures were generated with the RNAstructure, a fully automated miRNAs structure modelling server, accessible via the Web Servers for RNA Secondary Structure Prediction.ResultsWe demonstrated a search for nucleotide sequence and the projection of the molecular structure of the following miRNA inhibitors against miRNA over-expressed in thyroid cancer: miRNA-101, miRNA-126, miRNA-126-3p, miRNA-141, miRNA-145, miRNA-146b, miRNA-206, miRNA-3666, miRNA-497, miRNA-539, miRNA-613, and miRNA-618.ConclusionIn this study we show in silico secondary structures projection of selected of 12 miRNA inhibitors against miRNA over-expressed in thyroid cancer through computational biology.