De novo genome assembly and functional annotation forFusarium langsethiae

Abstract

AbstractMotivationFusarium langsethiaeis a T-2 and HT-2 mycotoxins producingFusariumspecies firstly characterised in 2004. It is commonly isolated from oats in Northern Europe. T-2 and HT-2 mycotoxins exhibit immunological and haemotological effects in animal health mainly through inhibition of protein, RNA and DNA synthesis. The development of a high-quality and comprehensively annotated assembly for this species is therefore essential in providing the molecular understanding and the mechanism of T-2 and HT-2 biosynthesis inF. langsethiaeto help develop effective control strategies.ResultsTheF. langsethiaeassembly was produced using PacBio long reads, which were then assembled independently using Canu, SMARTdenovo and Flye; producing a genome assembly total length of 59Mb and N50 of 3.51Mb. A total of 19,336 coding genes were identified using RNA-Seq informedab-initiogene prediction. Finally, predicting genes were annotated using the basic local alignment search tool (BLAST) against the NCBI non-redundant (NR) genome database and protein hits were annotated using InterProScan. Genes with blast hits were functionally annotated with Gene Ontology.Contactf.mohareb@cranfield.ac.ukData availabilityRaw sequence reads and assembled genome can be downloaded from: GenBank under the accession JAFFKB000000000