Layer 3 dynamically coordinates columnar activity according to spatial context

Abstract

AbstractSpatial integration is a fundamental, context-dependent neural operation that involves extensive neural circuits across cortical layers of V1. To better understand how spatial integration is dynamically coordinated across layers we recorded single- and multi-unit activity and local field potentials across V1 layers of awake mice, and used dynamic Bayesian model comparisons to identify when laminar activity and inter-laminar functional interactions showed surround suppression, the hallmark of spatial integration. We found that surround suppression is strongest in layer 3 (L3) and L4 activity, showing rapidly sharpening receptive fields and increasing suppression strength. Importantly, we also found that specific directed functional connections were strongest for intermediate stimulus sizes and suppressed for larger ones, particularly for the L3->L5 and L3->L1 connections. Taken together, the results shed light on the different functional roles of cortical layers in spatial integration and show how L3 dynamically coordinates activity across a cortical column depending on spatial context.