Construction of an ∼700-kb Transcript Map Around the Familial Mediterranean Fever Locus on Human Chromosome 16p13.3

Author:

Centola Michael,Chen Xiaoguang,Sood Raman,Deng Zuoming,Aksentijevich Ivona,Blake Trevor,Ricke Darrell O.,Chen Xiang,Wood Geryl,Zaks Nurit,Richards Neil,Krizman David,Mansfield Elizabeth,Apostolou Sinoula,Liu Jingmei,Shafran Neta,Vedula Anil,Hamon Melanie,Cercek Andrea,Kahan Tanaz,Gumucio Deborah,Callen David F.,Richards Robert I.,Moyzis Robert K.,Doggett Norman A.,Collins Francis S.,Liu P. Paul,Fischel-Ghodsian Nathan,Kastner Daniel L.

Abstract

We used a combination of cDNA selection, exon amplification, and computational prediction from genomic sequence to isolate transcribed sequences from genomic DNA surrounding the familial Mediterranean fever (FMF) locus. Eighty-seven kb of genomic DNA around D16S3370, a marker showing a high degree of linkage disequilibrium with FMF, was sequenced to completion, and the sequence annotated. A transcript map reflecting the minimal number of genes encoded within the ∼700 kb of genomic DNA surrounding the FMF locus was assembled. This map consists of 27 genes with discreet messages detectable on Northerns, in addition to three olfactory-receptor genes, a cluster of 18 tRNA genes, and two putative transcriptional units that have typical intron–exon splice junctions yet do not detect messages on Northerns. Four of the transcripts are identical to genes described previously, seven have been independently identified by the French FMF Consortium, and the others are novel. Six related zinc-finger genes, a cluster of tRNAs, and three olfactory receptors account for the majority of transcribed sequences isolated from a 315-kb FMF central region (betweenD16S468/D16S3070 and cosmid 377A12). Interspersed among them are several genes that may be important in inflammation. This transcript map not only has permitted the identification of the FMF gene (MEFV), but also has provided us an opportunity to probe the structural and functional features of this region of chromosome 16.

Publisher

Cold Spring Harbor Laboratory

Subject

Genetics (clinical),Genetics

Reference97 articles.

1. Initial assessment of human gene diversity and expression patterns based upon 83 million nucleotides of cDNA sequence.;Adams;Nature,1995

2. Familial Mediterranean fever (FMF) in Moroccan Jews: Demonstration of a founder effect by extended haplotype analysis.;Aksentijevich;Am. J. Hum. Genet.,1993

3. Human ICE/CED-3 Protease Nomenclature

4. A novel G protein-coupled receptor kinase gene cloned from 4p16.3

Cited by 16 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3