Abstract
AbstractComplementing whole genome sequencing strategies with high-throughput multiplex RT-qPCR genotyping allows for more comprehensive and real-time tracking of SARS-CoV-2 variants of concern. During the second and third waves of COVID-19 in Qatar, PCR genotyping, combined with Sanger sequencing of un-typeable samples, was employed to describe the epidemiology of the Alpha, Beta and Delta variants. A total of 9792 nasopharyngeal PCR-positive samples collected between April-June 2021 were successfully genotyped, revealing the importation and transmission dynamics of these three variants in Qatar.
Publisher
Cold Spring Harbor Laboratory
Reference16 articles.
1. WHO. Tracking SARS-CoV-2 variants 2021 [Available from: https://www.who.int/en/activities/tracking-SARS-CoV-2-variants/.
2. Abu-Raddad LJ , Chemaitelly H , Butt AA , National Study Group for Covid-19 Vaccination. Effectiveness of the BNT162b2 Covid-19 Vaccine against the B.1.1.7 and B.1.351 Variants. N Engl J Med. 2021.
3. Chemaitelly H , Yassine HM , Benslimane FM , Al Khatib HA , Tang P , Hasan MR , et al. mRNA-1273 COVID-19 vaccine effectiveness against the B.1.1.7 and B.1.351 variants and severe COVID-19 disease in Qatar. Nat Med. 2021.
4. WHO. Methods for the detection and identification of SARS-CoV-2 variants 2021 [Available from: https://apps.who.int/iris/bitstream/handle/10665/340067/WHO-EURO-2021-2148-41903-57493-eng.pdf?sequence=1&isAllowed=y.
5. Vogels C , Fauver J , Grubaugh N. Multiplexed RT-qPCR to screen for SARS-COV-2 B.1.1.7, B.1.351, and P.1 variants of concern V.3. protocolsio. 2021.
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