Collective Mechanical Responses of Cadherin-Based Adhesive Junctions as Predicted by Simulations

Abstract

ABSTRACTCadherin-based adherens junctions and desmosomes help stabilize cell-cell contacts with additional function in mechano-signaling, while clustered protocadherin junctions are responsible for directing neuronal circuits assembly. Structural models for adherens junctions formed by epithelial cadherin (CDH1) proteins indicate that their long, curved ectodomains arrange to form a periodic, two-dimensional lattice stabilized by tip-to-tip trans interactions (across junction) and lateral cis contacts. Less is known about the exact architecture of desmosomes, but desmoglein (DSG) and desmocollin (DSC) cadherin proteins are also thought to form ordered junctions. In contrast, clustered protocadherin (PCDH) based cell-cell contacts in neuronal tissues are thought to be responsible for self-recognition and avoidance, and structural models for clustered PCDH junctions show a linear arrangement in which their long and straight ectodomains form antiparallel overlapped trans complexes. Here we report all-atom molecular dynamics simulations testing the mechanics of minimalistic adhesive junctions formed by CDH1, DSG2 coupled to DSC1, and PCDHγB4, with systems encompassing up to 3.7 million atoms. Simulations generally predict a favored shearing pathway for the adherens junction model and a two-phased elastic response to tensile forces for the adhesive adherens junction and the desmosome models. Complexes within these junctions first unbend at low tensile force and then become stiff to unbind without unfolding. However, cis interactions in both the CDH1 and DSG2-DSC1 systems dictate varied mechanical responses of individual dimers within the junctions. Conversely, the clustered protocadherin PCDHγB4 junction lacks a distinct two-phased elastic response. Instead, applied tensile force strains trans interactions directly as there is little unbending of monomers within the junction. Transient intermediates, influenced by new cis interactions, are observed after the main rupture event. We suggest that these collective, complex mechanical responses mediated by cis contacts facilitate distinct functions in robust cell-cell adhesion for classical cadherins and in self-avoidance signaling for clustered PCDHs.Statement of SignificanceProteins that mediate cell-cell contacts often form aggregates in vivo where the tight packing of monomers into junctions is relevant to their function. Members of the cadherin superfamily of glycoproteins form large complexes in which their long ectodomains interact to mediate cell-cell adhesion. Here, we employ simulations to elucidate complex mechanical responses of five junction systems in response to force. Our results offer atomistic insights into the behavior of these proteins in a crowded physiological context, suggesting that classical cadherin complexes in adherens junctions and desmosomes act as molecular shock absorbers with responses modulated by dynamic lateral contacts, while clustered protocadherins form brittle junctions that upon stretching and unbinding form transient interfaces suitable for their critical role in neuronal self-recognition.