Cytoplasmic organization promotes protein diffusion

Abstract

ABSTRACTThe cytoplasm is highly organized. However, the extent to which this organization influences the dynamics of cytoplasmic proteins is not well understood. Here, we used Xenopus laevis egg extracts as a model system to study diffusion dynamics in organized versus disorganized cytoplasm. Such extracts are initially homogenized and disorganized, and will self-organize into cell-like units over the course of 20-60 min. Using fluorescence correlation spectroscopy, we observed that self-organization is accompanied by changes in protein diffusivity; as the extract organizes, proteins diffuse about twice as quickly over a length scale of a few hundred nanometers. Even though the ordered cytoplasm contained organelles and cytoskeletal elements that might be expected to interfere with diffusion, after self-organization took place, the speed of protein diffusion approached that of organelle-depleted cytosolic extracts. This finding suggests that subcellular organization optimizes protein diffusivity. The effect of organization on diffusion varies with molecular size, with the effects being largest for protein-sized molecules. These results show that cytoplasmic organization promotes the efficient diffusion of protein molecules in a densely packed environment.