Functional connectomics spanning multiple areas of mouse visual cortex
Author:
, Bae J. AlexanderORCID, Baptiste Mahaly, Bodor Agnes L., Brittain Derrick, Buchanan JoAnnORCID, Bumbarger Daniel J., Castro Manuel A., Celii Brendan, Cobos Erick, Collman Forrest, da Costa Nuno MaçaricoORCID, Dorkenwald SvenORCID, Elabbady Leila, Fahey Paul G.ORCID, Fliss Tim, Froudarakis Emmanouil, Gager Jay, Gamlin Clare, Halageri Akhilesh, Hebditch James, Jia Zhen, Jordan Chris, Kapner Daniel, Kemnitz NicoORCID, Kinn Sam, Koolman Selden, Kuehner Kai, Lee Kisuk, Li Kai, Lu Ran, Macrina ThomasORCID, Mahalingam Gayathri, McReynolds Sarah, Miranda Elanine, Mitchell Eric, Mondal Shanka Subhra, Moore Merlin, Mu Shang, Muhammad Taliah, Nehoran Barak, Ogedengbe Oluwaseun, Papadopoulos Christos, Papadopoulos SteliosORCID, Patel Saumil, Pitkow XaqORCID, Popovych Sergiy, Ramos Anthony, Reid R. Clay, Reimer Jacob, Schneider-Mizell Casey M.ORCID, Seung H. SebastianORCID, Silverman Ben, Silversmith WilliamORCID, Sterling Amy, Sinz Fabian H., Smith Cameron L.ORCID, Suckow Shelby, Takeno MarcORCID, Tan Zheng H., Tolias Andreas S., Torres Russel, Turner Nicholas L.ORCID, Walker Edgar Y.ORCID, Wang TianyuORCID, Williams GraceORCID, Williams Sarah, Willie Kyle, Willie Ryan, Wong William, Wu JingpengORCID, Xu Chris, Yang RunzheORCID, Yatsenko Dimitri, Ye Fei, Yin Wenjing, Yu Szi-chieh
Abstract
ABSTRACTThe value of an integrated approach for understanding the neocortex by combining functional characterization of single neuron activity with the underlying circuit architecture has been understood since the dawn of modern neuroscience. However, in practice, anatomical connectivity and physiology have been studied mostly separately. Following in the footsteps of previous studies that have combined physiology and anatomy in the same tissue, here we present a unique functional connectomics dataset that contains calcium imaging of an estimated 75,000 neurons from primary visual cortex (VISp) and three higher visual areas (VISrl, VISal and VISlm), that were recorded while a mouse viewed natural movies and parametric stimuli. The functional data were co-registered with electron microscopy (EM) data of the same volume which were automatically segmented, reconstructing more than 200,000 cells (neuronal and non-neuronal) and 524 million synapses. Subsequent proofreading of some neurons in this volume yielded reconstructions that include complete dendritic trees as well the local and inter-areal axonal projections. The largest proofread excitatory axon reached a length of 19 mm and formed 1893 synapses, while the largest inhibitory axon formed 10081 synapses. Here we release this dataset as an open access resource to the scientific community including a set of analysis tools that allows easy data access, both programmatically and through a web user interface.
Publisher
Cold Spring Harbor Laboratory
Cited by
71 articles.
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