Genetics and Gene Editing Methods inXenopus laevisandXenopus tropicalis

Abstract

Our understanding of biological systems has for many years been heavily influenced by experimental approaches that exploit genetic methods. These include gain-of-function experiments that overexpress transgenes or ectopically express injected RNA and loss-of-function experiments that knock out genes or knock down RNAs. Here, we review how these methods have been applied inXenopusfrogs and introduce a variety of protocols for genetic manipulation ofXenopus laevisandXenopus tropicalis.