The wheat Sr22, Sr33, Sr35 and Sr45 genes confer resistance against stem rust in barley
Author:
Asyraf Md. Hatta M.ORCID, Johnson Ryan, Matny Oadi, Smedley Mark A., Yu Guotai, Chakraborty Soma, Bhatt Dhara, Xia Xiaodi, Arora Sanu, Steuernagel BurkhardORCID, Richardson Terese, Mago RohitORCID, Lagudah Evans S.ORCID, Patron NicolaORCID, Ayliffe Mick, Rouse Matthew N., Harwood Wendy A.ORCID, Periyannan Sambasivam K., Steffenson Brian J.ORCID, Wulff Brande B. H.ORCID
Abstract
SummaryIn the last 20 years, stem rust caused by the fungus Puccinia graminis f. sp. tritici (Pgt), has re-emerged as a major threat to wheat and barley cultivation in Africa and Europe. In contrast to wheat with 82 designated stem rust (Sr) resistance genes, barley’s genetic variation for stem rust resistance is very narrow with only seven resistance genes genetically identified. Of these, only one locus consisting of two genes is effective against Ug99, a strain of Pgt which emerged in Uganda in 1999 and has since spread to much of East Africa and parts of the Middle East. The objective of this study was to assess the functionality, in barley, of cloned wheat Sr genes effective against Ug99. Sr22, Sr33, Sr35 and Sr45 were transformed into barley cv. Golden Promise using Agrobacterium-mediated transformation. All four genes were found to confer effective stem rust resistance. The barley transgenics remained susceptible to the barley leaf rust pathogen Puccinia hordei, indicating that the resistance conferred by these wheat Sr genes was specific for Pgt. Cloned Sr genes from wheat are therefore a potential source of resistance against wheat stem rust in barley.
Publisher
Cold Spring Harbor Laboratory
Reference93 articles.
1. Indirect activation of a plant nucleotide binding site-leucine-rich repeat protein by a bacterial protease 2. Allele characterization of genes required for rpg4-mediated wheat stem rust resistance identifies Rpg5 as the R gene;Phytopathology,2013 3. Arora, S. , Steuernagel, B. , Chandramohan, S. , Long, Y. , Matny, O. , Johnson, R. , Enk, J. , Periyannan, S. , Hatta, M.A.M. , Athiyannan, N. , Cheema, J. , Yu, G. , Kangara, N. , Ghosh, S. , Szabo, L.J. , Poland, J. , Bariana, H. , Jones, J.D.G. , Bentley, A.R. , Ayliffe, M. , Olson, E. , Xu, S.S. , Steffenson, B.J. , Lagudah, E. and Wulff, B.B.H. (2018) Resistance gene discovery and cloning by sequence capture and association genetics. bioRxiv. 4. Durable resistance to wheat stem rust needed 5. NLR diversity, helpers and integrated domains: making sense of the NLR IDentity
Cited by
5 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献
|
|