Exploring novel probiotic mechanisms ofStreptococcusA12 with functional genomics

Abstract

AbstractHealth-associated biofilms in the oral cavity are composed of a diverse group of microbial species that can foster an environment that is less favorable for the outgrowth of dental caries pathogens, likeStreptococcus mutans.A novel oral bacterium, designatedStreptococcusA12, was previously isolated from supragingival dental plaque of a caries-free individual, and was shown to interfere potently with the growth and virulence properties ofS. mutans. Here, we apply functional genomics to begin to identify molecular mechanisms used by A12 to antagonize, and to resist the antagonistic factors of,S. mutans.Using bioinformatics, genes that could encode factors that enhance the ability of A12 to compete withS. mutanswere identified. Selected genes, designated aspotentialcompetitive factors (pcf), were deleted. Certain mutant derivatives showed a reduced capacity to compete withS. mutanscompared to the parental strain. The A12pcfOmutant lost the ability to inhibitcomX-inducingpeptide (XIP) signaling byS. mutans, while mutants in thepcfFEGlocus were impaired in sensing of, and were more sensitive to, the lantibiotic nisin. Loss of PcfV, annotated as a colicin V biosynthetic protein, resulted in diminished antagonism ofS. mutans.Collectively, the data provide new insights into the complexities and variety of factors that affect biofilm ecology and virulence. Continued exploration of the genomic and physiologic factors that distinguish commensals from truly beneficial members of the oral microbiota will lead to a better understanding of the microbiome and new approaches to promote oral health.ImportanceAdvances in defining the composition of health-associated biofilms have highlighted the important role for beneficial species in maintaining health. Comparatively little, however, has been done to address the genomic and physiological basis underlying the probiotic mechanisms of beneficial commensals. In this study, we explored the ability of a novel oral bacterial isolate,StreptococcusA12, to compete with the dental pathogenStreptococcus mutans, using various gene products with diverse functions. A12 displayed enhanced competitiveness by: i) disrupting intercellular communication pathways ofS. mutans, ii) sensing and resisting antimicrobial peptides, and iii) producing factors involved in the production of a putative antimicrobial compound. Research on the probiotic mechanisms employed byStreptococcusA12 is providing essential insights into how beneficial bacteria may help maintain oral health, which will aid in the development of biomarkers and therapeutics that can improve the practice of clinical dentistry.