Optimization of Alginante-poly-L-lysine Microencapsules Strength by Box-Behnken Model for CHO cells Culture in Stirred Tank Bioreactor

Abstract

Summary statementBox-Behnken model is an efficient method to optimized process conditions of microencapsules strength for long-term cell culture, promotes CHO cells viability and protein production in a stirred tank bioreactor.ABSTRACTCell microencapsulation technology has been proved to be a valuable technology in the fields of large-scale cell culture. It is important to accurately construct the microcapsule membranes with desired properties including a certain thickness suitable for cell growth, and maximum strength for the stability of microencapsules. As single factor experiments are time-consuming to obtain the desired membrane preparation conditions, Box-Behnken model was used to investigate the interactions among reaction conditions, predict the optimized reaction conditions for given purpose, that was membrane with maximum strength and desired thickness for microencapsulated cell culture. Significant values of R2 in this study indicated the model theoretical values are very close to the measured values. Based on the desired membrane thickness, the process of maximum strength was optimized, and the prediction agreement of measured value and model theoretical value was 91.11%. The optimized microencapsules with maximum strength and 15 μm membrane thickness promote CHO cells viability and protein production in stirred tank bioreactor. The result shows that Box-Behnken model is an efficient method to optimized process conditions of microencapsules strength for long-term cell culture.