Identification of Thermotolerant Rice Genotypes with Allele Coding at Seedling Stage

Abstract

AbstractRice-The most important plant in the world to ensure food security. Heat is one of the main factors that greatly limit rice production. With the increasing global warming, industrialization there is a great effect on climate change which requires us to see various alternatives for strains that are more tolerant to heat so that some techniques are developed to filter a large number of genotypes for high temperature tolerance. Here we report the standardization of Temperature Induction Response (TIR) technique to identify thermotolerant rice genotypes. The phenotypic characteristics of Rice due to high temperature is calculated with germination (%), growth of the seedling and molecular analysis is also considered. The heat stress is provided to the plants with the help of TIR protocol with the adjustment of temperature to lethal (55°C) and sub-lethal levels (38-55°C) in a TIR chamber with alterations in humidity. Of the 74 genotypes screened, 14 showed thermo tolerance caused by high temperatures. Both tolerant and sensitive genotypes were separated based on their survival percentages. The tolerant class are selected based on the growth and development of genotypes having high survival percentage and also their shoot and root lengths, fresh and dry weights are compared to the heat tolerant checks N22, Dular and Nipponbare. These genotypes have intrinsic heat tolerance and thus can be explored as a source of donors in breeding programs intended for global warming. The molecular markers which are identified to be linked with heat tolerant class through allele code are quite helpful and can be used in marker assisted breeding approach to attain heat tolerance in cultivated varieties.