Identification and assessment of the antibacterial activity of ZnO nanoparticles produced by Vigna mungo and Rhizobacteria

Abstract

1.ABSTRACTSZnO nanoparticles have received a lot of interest in recent years due to their unusual features. Antimicrobial properties of ZnO NPs However, the qualities of nanoparticles are determined by their size and form, making them suitable for a variety of applications. The current work looks at the synthesis, characterization, and antibacterial activity of ZnO NPs produced by Vigna Mungo and Rhizobacteria. Rhizobacteria isolated from V. mungo root nodule were morphologically, biochemically, and molecularly examined and identified as Rhizobium sp. strain P4 and Bacillus flexus strain IFO15715. The GC-MS analysis of methanol leaf extract of V. mungo was performed to detect and identify bioactive chemicals, and this indicated phytol as an antibacterial agent, while Squalene and Alpha – tocopherol had antioxidant and anti-tumour properties. Agar well diffusion experiment was used to determine the antibacterial properties of ZnO nanoparticles and Vigna Mungo leaf extract. This approach is widely documented, and standard zones of inhibition for sensitive and resistant values have been defined. The results demonstrated that both methanol extract and zinc oxide nanoparticles have strong antibacterial efficacy against the majority of the pathogens examined. he synthesized nanoparticles from Rhizobium sp. were characterized by analytical techniques like SEM, XRD, FTIR, and UV-Vis.