Abstract
AbstractHi-C, a genome-wide chromosome conformation capture assay is a powerful tool used to study three-dimensional genome organisation by converting physical pairwise interactions into counts of pairwise interaction. To study the many temporally regulated facets of meiotic recombination inS. cerevisiaethe Hi-C assay must be robust such that fine- and wide-scale comparisons between genetic datasets can be made. Here we describe an updated protocol for Hi-C (Hi-C2B) that generates reproducible libraries of interaction data with low noise and for a relatively low cost.
Publisher
Cold Spring Harbor Laboratory