Metabolic Regulation of Single Synaptic Vesicle Exo- and Endocytosis in Hippocampal Synapses

Author:

Myeong JongyunORCID,Stunault Marion IORCID,Klyachko Vitaly AORCID,Ashrafi GhazalehORCID

Abstract

SUMMARYGlucose has long been considered a primary source of energy for synaptic function. However, it remains unclear under what conditions alternative fuels, such as lactate/pyruvate, contribute to powering synaptic transmission. By detecting individual release events in cultured hippocampal synapses, we found that mitochondrial ATP production from oxidation of lactate/pyruvate regulates basal vesicle release probability and release location within the active zone (AZ) evoked by single action potentials (APs). Mitochondrial inhibition shifted vesicle release closer to the AZ center, suggesting that the energetic barrier for vesicle release is lower in the AZ center that the periphery. Mitochondrial inhibition also altered the efficiency of single AP evoked vesicle retrieval by increasing occurrence of ultrafast endocytosis, while inhibition of glycolysis had no effect. Mitochondria are sparsely distributed along hippocampal axons and we found that nerve terminals containing mitochondria displayed enhanced vesicle release and reuptake during high-frequency trains, irrespective of whether neurons were supplied with glucose or lactate. Thus, synaptic terminals can entirely bypass glycolysis to robustly maintain the vesicle cycle using oxidative fuels in the absence of glucose. These observations further suggest that mitochondrial metabolic function not only regulates several fundamental features of synaptic transmission but may also contribute to modulation of short-term synaptic plasticity.HighlightsSynapses can sustain neurotransmission across various activity levels by bypassing glycolysis and utilizing oxidative fuels.Mitochondria, but not glycolysis, regulate release probability and nanoscale organization of vesicle release within the active zone.Mitochondrial inhibition increases the occurrence of vesicle retrieval via ultra-fast endocytosis.Mitochondrial localization in nerve terminals enhances vesicle release and retrieval in the absence of glucose, representing a form of synaptic plasticity.

Publisher

Cold Spring Harbor Laboratory

Reference62 articles.

1. [The glucose consumption of the brain & its dependence on the liver];Arch. Psychiatr. Nervenkrankh. Ver. Mit Z. Gesamte Neurol. Psychiatr,1958

2. Relationship among Brain and Blood Glucose Levels and Spontaneous and Glucoprivic Feeding

3. Fioramonti, X. , Chrétien, C. , Leloup, C. & Pénicaud, L . Recent Advances in the Cellular and Molecular Mechanisms of Hypothalamic Neuronal Glucose Detection. Front Physiol 8, (2017).

4. Extracellular Glucose Concentrations in the Rat Hippocampus Measured byZero-Net-Flux

5. Mitochondria in Neuronal Health: From Energy Metabolism to Parkinson’s Disease. Adv;Biol,2021

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