Abstract
ABSTRACTWe describe five bacterial isolates that were isolated fromFragaria x ananassain 1976 in Rydalmere, Australia, during routine biosecurity surveillance. Initially, biochemical characterisation identified these isolates as members of theXanthomonasgenus. To determine their species, we conducted further analysis using both phenotypic and genotypic approaches. Phenotypic analysis involved using MALDI-TOF MS and BIOLOG GEN III microplates, which confirmed that the isolates belonged to theXanthomonasgenus but could not classify species. Genome relatedness indices and extensive phylogenetic analysis confirmed that the isolates belonged to theXanthomonasgenus and represented a new species. Based on the absence of virulence factors typically found inXanthomonasspp. genomes, we suggest that these isolates are non-pathogenic. This conclusion was supported by a pathogenicity assay. Based on these findings, we propose the nameXanthomonas rydalmerenesis, with DAR34855 = ICMP24941 as the type strain.
Publisher
Cold Spring Harbor Laboratory
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