Prevalence of TEM and SHV Genes inKlebsiella pneumoniaeisolated from different Clinical specimens in Khartoum, Sudan

Abstract

AbstractObjectiveThe aim of this study is to determine the prevalence of ESBL producingKlebsiella pneumoniaeisolated from different clinical specimens gathered from different clinics in Khartoum State.Settings and DesignThis is a descriptive cross-sectional study conducted in Khartoum State during a period from February to May 2018.Methods and MaterialsKlebsiella pneumoniaeisolated from patients attending to health-care settings with different infections, which was re-identiGied by conventional methods and screened for ESBL production by modiGied Kirby-Bauer’s method. The TEM and SHV genes were detected by PCR.ResultsOut of 100K. pneumoniaeisolates, 85% (85/100) were ESBL producing strains. The highest prevalence of ESBL production was in blood 100% (6/6) as well as stool 100% (4/4) followed by sputum 91% (21/23), wound 85% (12/14) and urine 82%(40\49) samples, while the lowest was noted in CSF 50% (2/4). The isolatedK. pneumoniaeshowed high resistance to Ceftazidime 85% followed by Cefotaxime and Cefpodoxime 80% for each and 73% for Aztreonam. Furthermore, we found that 88% of isolates were resistant to Colistin and 33% were resistant to Meropenem. TEM and SHV genes were detected in 9.4% (8/85) and 1.2% (1/85) of ESBL producing strains, respectively. However, SHV was also detected in 6.7% (1/15) of non-ESBL-producingK. pneumoniae.ConclusionsA high prevalence of ESBL-producingK. pneumoniaewas detected in this study Which is 85%. TEM and SHV genes were found in some strains of ESBL producingK. pneumoniae. The Gindings of this study alarm the physicians to take ESBL producing strains ofK. pneumoniaeunder consideration before making treatment decisions.