Abstract
AbstractWe report a workflow based on ozone-induced dissociation for untargeted characterization of hundreds ofsn-resolved glycerophospholipid isomers from biological extracts in under 20 minutes, coupled with an automated data analysis pipeline. It provides an order of magnitude increase in the number ofsn-isomer pairs identified compared to previous reports, reveals thatsn-isomer populations are tightly regulated and significantly different between cell lines, and enables identification of rare lipids containing ultra-long chain monounsaturated acyl chains.
Publisher
Cold Spring Harbor Laboratory