Author:
Bharadwaj Revuru,Thashanamoorthi Gayathri,Demiwal Pratibha,Sircar Debabrata,Ramalingam Sathishkumar
Abstract
SummaryCarnosol (CO) and carnosic acid (CA) are pharmaceutically important diterpenes predominantly produced in members of Lamiaceae,Salvia officinalis,Salvia fruticosaandRosmarinus officinalis. Nevertheless, availability of these compounds in plant system is very low.With an effort to improve thein plantacontent of these diterpenes,SmERF6(Salvia miltiorrhiza Ethylene Responsive Factor 6) transcription factor was expressed inS. officinalisheterologously. SmERF6 is known to bind at the promoter regions ofCopalyl diphosphate synthase(CPS) andKaurene synthase like(KSL) genes and improve ferruginol content, a common precursor for abietane diterpenes inSalviagenus.Transient expression ofSmERF6exhibited the inter-specific activity in promoting differential accumulation of diterpenes inS. officinalis. Overexpression studies showed elevation in the levels of CO (10-folds) and CA (8-folds). Further, in infiltrated leaves levels of ferruginol (50%) and CA derivatives (rosmanol, epirosmanal, methyl carnosic acid) were significantly upregulated along with the other signature terpenes. While, knockdown of homologousERF6resulted in drastic reduction of the metabolite content.Finally, stable transgenic lines ofS. officinalisdeveloped throughin planta Agrobacteriummediated genetic transformation method accumulated higher levels of CO (4-folds) and CA (3-folds) as compared to wild plants.Overall, the present study is the first report on improving the content of pharmaceutically important diterpenes inS. officinalisby overexpressing pathway specific transcription factor. The current results showed convincing evidence for the concept of improving the content of specialized metabolite(s) in medicinal plants by manipulating the expression of key transcription factors.
Publisher
Cold Spring Harbor Laboratory