Author:
Chen Hongbo,Xie Zhongping,Long Runxiang,Fan Shengtao,Li Heng,He Zhanlong,Xu Kanwei,Liao Yun,Wang Lichun,Zhang Ying,Li Xueqi,Dong Xingqi,Mou Tangwei,Zhou Xiaofang,Yang Yaoyun,Guo Lei,Yang Jianbo,Zheng Huiwen,Xu Xingli,Li Jing,Liang Yan,Li Dandan,Zhao Zhimei,Hong Chao,Zhao Heng,Jiang Guorun,Che Yanchun,Yang Fengmei,Hu Yunguang,Wang Xi,Pu Jing,Ma Kaili,Wang Lin,Chen Chen,Duan Weiguo,Shen Dong,Zhao Hongling,Jiang Ruiju,Deng Xinqiang,Li Yan,Zhu Hailian,Zhou Jian,Yu Li,Xu Mingjue,Yang Huijuan,Yi Li,Zhou Zhenxin,Yang Jiafang,Duan Nan,Yang Huan,Zhao Wangli,Yang Wei,Li Changgui,Liu Longding,Li Qihan
Abstract
AbstractWith the relatively serious global epidemic outbreak of SARS-CoV-2 infection, public concerns focus on not only clinical therapeutic measures and public quarantine for this disease but also the development of vaccines. The technical design of our SARS-CoV-2 inactivated vaccine provides a viral antigen that enables the exposure of more than one structural protein based upon the antibody composition of COVID-19 patients’ convalescent serum. This design led to valid immunity with increasing neutralizing antibody titers and a CTL response detected post-immunization of this vaccine by two injections in rhesus macaques. Further, this elicited immunoprotection in macaques enables not only to restrain completely viral replication in tissues of immunized animals, compared to the adjuvant control and those immunized by an RBD peptide vaccine, but also to significantly alleviate inflammatory lesion in lung tissues in histo-pathologic detection, compared to the adjuvant control with developed interstitial pneumonia. The data obtained from these macaques immunized with the inactivated vaccine or RBD peptide vaccine suggest that immunity with a clinically protective effect against SARS-CoV-2 infection should include not only specific neutralizing antibodies but also specific CTL responses against at least the S and N antigens.
Publisher
Cold Spring Harbor Laboratory
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