Extraction of accurate cytoskeletal actin velocity distributions from noisy measurements

Abstract

Dynamic remodeling of the actin cytoskeleton is essential for many cellular processes. Tracking the movement of individual actin filaments can in principle shed light on how this complex behavior arises at the molecular level. However, the information that can be extracted from these measurements is often limited by low signal-to-noise ratios. We developed a Bayesian statistical approach to estimate true, underlying velocity distributions from the tracks of individual actin-associated fluorophores with quantified localization uncertainties. We found that filamentous (F)-actin velocity distributions in fibroblasts and endothelial cells were well described by a statistical jump process, in which filaments exist in mechanical equilibria punctuated by abrupt, jump-like movements. A model with exponentially distributed jump length- and time-scales recapitulated actin filament velocity distributions measured for the cell cortex, integrin-based adhesions, and stress fibers, suggesting that a common physical model can potentially describe actin filament dynamics in a variety of cellular contexts.