Author:
Kvedaraite Egle,Hertwig Laura,Sinha Indranil,Ponzetta Andrea,Myrberg Ida Hed,Lourda Magda,Dzidic Majda,Akber Mira,Klingström Jonas,Folkesson Elin,Muvva Jagadeeswara Rao,Chen Puran,Brighenti Susanna,Norrby-Teglund Anna,Eriksson Lars I.,Rooyackers Olav,Aleman Soo,Strålin Kristoffer,Ljunggren Hans-Gustaf,Ginhoux Florent,Björkström Niklas K.,Henter Jan-Inge,Svensson Mattias,
Abstract
ABSTRACTMonocytes and dendritic cells are crucial mediators of innate and adaptive immune responses during viral infection, but misdirected responses by these cells might contribute to immunopathology. A comprehensive map of the mononuclear phagocyte (MNP) landscape during SARS-CoV-2 infection and concomitant COVID-19 disease is lacking. We performed 25-color flow cytometry-analysis focusing on MNP lineages in SARS-CoV-2 infected patients with moderate and severe COVID-19. While redistribution of monocytes towards intermediate subset and decrease in circulating DCs occurred in response to infection, severe disease associated with appearance of Mo-MDSC-like cells and a higher frequency of pre-DC2. Furthermore, phenotypic alterations in MNPs, and their late precursors, were cell-lineage specific and in select cases associated with severe disease. Finally, unsupervised analysis revealed that the MNP profile, alone, could identify a cluster of COVID-19 non-survivors. This study provides a reference for the MNP response to clinical SARS-CoV-2 infection and unravel myeloid dysregulation associated with severe COVID-19.
Publisher
Cold Spring Harbor Laboratory
Cited by
18 articles.
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