Expression Level Analysis of ACE2 Receptor Gene in African-American and Non-African-American COVID-19 Patients

Abstract

AbstractBackgroundThe COVID-19 pandemic caused by SARS-CoV-2 has spread rapidly across the continents. While the incidence of COVID-19 has been reported to be higher among African-American individuals, the rate of mortality has been lower compared to that of non-African-Americans. ACE2 is involved in COVID-19 as SARS-CoV-2 uses the ACE2 enzyme to enter host cells. Although the difference in COVID-19 incidence can be explained by many factors such as low accessibility of health insurance among the African-American community, little is known about ACE2 expression in African-American COVID-19 patients compared to non-African-American COVID-19 patients. The variable expression of genes can contribute to this observed phenomenon.MethodologyIn this study, transcriptomes from African-American and non-African-American COVID-19 patients were retrieved from the sequence read archive and analyzed for ACE2 gene expression. HISAT2 was used to align the reads to the human reference genome, and HTseq-count was used to get raw gene counts. EdgeR was utilized for differential gene expression analysis, and enrichR was employed for gene enrichment analysis.ResultsThe datasets included 14 and 33 transcriptome sequences from COVID-19 patients of African-American and non-African-American descent, respectively. There were 24,092 differentially expressed genes, with 7,718 upregulated (log fold change > 1 and FDR 0.05) and 16,374 downregulated (log fold change −1 and FDR 0.05). The ACE2 mRNA level was found to be considerably downregulated in the African-American cohort (p-value = 0.0242, p-adjusted value = 0.038).ConclusionThe downregulation of ACE2 in the African-American cohort could indicate a correlation to the low COVID-19 severity observed among the African-American community.