Comparative Analysis of SARS-CoV-2 Antigenicity across Assays and in Human and Animal Model Sera
Author:
Mühlemann BarbaraORCID, Wilks Samuel H, Baracco Lauren, Bekliz Meriem, Carreño Juan Manuel, Corman Victor MORCID, Davis-Gardner Meredith E., Dejnirattisai Wanwisa, Diamond Michael S, Douek Daniel C., Drosten Christian, Eckerle Isabella, Edara Venkata-Viswanadh, Ellis Madison, Fouchier Ron A M, Frieman Matthew, Godbole Sucheta, Haagmans Bart, Halfmann Peter J, Henry Amy R, Jones Terry CORCID, Katzelnick Leah C, Kawaoka Yoshihiro, Kimpel Janine, Krammer Florian, Lai Lilin, Liu Chang, Lusvarghi Sabrina, Meyer Benjamin, Mongkolsapaya Juthathip, Montefiori David C, Mykytyn Anna, Netzl AntoniaORCID, Pollett Simon, Rössler Annika, Screaton Gavin R, Shen Xiaoying, Sigal Alex, Simon Viviana, Subramanian Rahul, Supasa Piyada, Suthar MehulORCID, Türeli Sina, Wang Wei, Weiss Carol D, Smith Derek JORCID
Abstract
AbstractThe antigenic evolution of SARS-CoV-2 requires ongoing monitoring to judge the immune escape of newly arising variants. A surveillance system necessitates an understanding of differences in neutralization titers measured in different assays and using human and animal sera. We compared 18 datasets generated using human, hamster, and mouse sera, and six different neutralization assays. Titer magnitude was lowest in human, intermediate in hamster, and highest in mouse sera. Fold change, immunodominance patterns and antigenic maps were similar among sera. Most assays yielded similar results, except for differences in fold change in cytopathic effect assays. Not enough data was available for conclusively judging mouse sera, but hamster sera were a consistent surrogate for human first-infection sera.
Publisher
Cold Spring Harbor Laboratory
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