An effectiveC. elegansCRISPR training module for high school and undergraduate summer research experiences in molecular biology

Abstract

ABSTRACTEngaging in research experiences as a high school or undergraduate student interested in science, technology, engineering, and mathematics (STEM) is pivotal for their academic and professional development. A structured teaching framework can help cultivate a student’s curiosity and passion for learning and research. In this study, an effective eight-week training program has been created that encompasses fundamental molecular biology principles and hands-on laboratory activities. This curriculum focuses on using clustered regularly interspaced short palindromic repeats (CRISPR) gene editing in theCaenorhabditis elegansmodel organism. Through pre- and post-program assessments, substantial enhancements in students’ molecular biology proficiency and enthusiasm for scientific exploration was observed. Overall, this diligently crafted training module that employsC. elegansas an educational tool to instruct inexperienced students has demonstrated its accessibility and ability to engage students in molecular biology and gene editing methodologies.