A Far-Red Fluorescent Probe to VisualizeStaphylococcus aureusin Patient Samples

Abstract

AbstractStaphylococcus aureus (S. aureus)is the leading bacterial cause of death in high-income countries and can cause invasive infections at various body sites. These infections are associated with prolonged hospital stays, a large economic burden, considerable treatment failure, and mortality rates. So far, there is only limited knowledge about the specific locations whereS. aureusresides in the human body during various infections. Hence, the visualization ofS. aureusholds significant importance in microbiological research. Herein, we report the development and validation of a far-red-fluorescent probe to detectS. aureusin human biopsies from deep-seated infections. This probe displays strong fluorescence and low background in human tissues, outperforming current tools forS. aureusdetection. Several applications are demonstrated, including fixed- and live-cell imaging, flow cytometry, and super-resolution bacterial imaging.Abstract Figure